ABSTRACT
@#Selenium is an essential microelement required as a nutrient by human organism to perform important biological functions, mainly in the form of selenocysteine. It plays an important role in improving human immunity and the myocardial nutritional blood flow, and preventing oxidative stress. Various ocular diseases are closely associated with selenium and selenoprotein due to the presence of high concentration of selenium. This article mainly aims to review the present research advance of biological functions of selenium and selenoprotein in ocular diseases, and provide some valuable reference for deeper study of selenium in human eyes.
ABSTRACT
Integration is a critical step in the retroviral life cycle.HIV-1 integrase is involved in the integration of HIV DNA into host chromosomal DNA and appears to have no functionally equivalent in human cells.It has become an attractive and rational target for selective anti-AIDS therapy.A random linear heptapeptides phage display library was panned on the recombinant HIV-1 integrase protein.After five rounds of panning,13 positive phage clones were selected and sequenced.Two consensus peptides(TPSHSSR and HPERATL) were chemically synthesized.The non-radioactive ELISA-based HIV-1 integrase assay showed that the synthetic peptides TPSHSSR and HPERATL were able to inhibit the 3'cleavage or strand transfer activity of HIV-1 integrase to some extent(IC50=(54.56?5.18) ?mol/L,IC50=(28.29?1.32) ?mol/L,respectively) .These heptapeptides could be used for developing new anti-HIV drug candidates,as well as for structural studies of the three-dimensional structure of the entire integrase molecule.